eISSN: 1897-4252
ISSN: 1731-5530
Kardiochirurgia i Torakochirurgia Polska/Polish Journal of Thoracic and Cardiovascular Surgery
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1/2014
vol. 11
 
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abstract:

Percentages of NKT cells in the tissues of patients with non-small cell lung cancer who underwent surgical treatment

Maria Pyszniak
,
Paweł Rybojad
,
Katarzyna Pogoda
,
Andrzej Jabłonka
,
Agnieszka Bojarska-Junak
,
Jacek Tabarkiewicz

Kardiochirurgia i Torakochirurgia Polska 2014; 11 (1): 34-39
Online publish date: 2014/04/03
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Introduction: Natural killer T (NKT) cells are involved in the antitumor response by direct cytotoxicity and indirectly through activation of effector cells. Recent studies have shown a relationship between the number and function of NKT cells and clinical out-comes. NKT cells seem to represent a promising tool for immunotherapy of cancer.

The aim of the study was to evaluate the distribution of NKT cells in peripheral blood, lymph nodes and tumor tissue of non-small cell lung cancer (NSCLC) patients, as well as development of the most efficient set of cytokines stimulating differentiation of NKT cells.

Material and methods: We evaluated the percentage of iNKT+CD3+ cells in the tissues collected from patients with NSCLC. For the generation of NKT cells, we cultured cells isolated from the blood of 20 healthy donors and from the tissues of 4 NSCLC patients. Cells were stimulated with α-GalCer in combinations with cytokines.

Results: We noted significant differences in the percentages of NKT cells in the patients’ tissues. The highest percentage of these cells was observed in the tumor tissue and the lowest in the lymph nodes. In vitro, in healthy donors all α-GalCer-cytokine com-binations were effective in stimulation of NKT cells’ proliferation. NKT cells’ proliferation was the most efficiently stimulated by α-GalCer+IL-2+IL-7 and α-GalCer+IL-2+IFN-γ.

Conclusions: Our results suggest that in the course of NSCLC, NKT cells migrate to the primary tumor and accumulate therein. All tested combinations of α-GalCer and cytokines were capable of generation of NKT cells in vitro.
keywords:

NSCLC, NKT, peripheral blood, lymph nodes, tumor

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