eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Newsletter
Enter your email:
 
Search
Editorial Policies
Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

Without publication fees

without publication fees

Open access

without publication fees

Journal integrated with

integrated with publons
4/2016
vol. 97
 
Share:
Send email
Share:
abstract:

Simultaneous fermentative chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-1-38 and their antifungal activity against rice sheath blight disease

Sawai Boukaew
1
,
Wanida Petlamul
1
,
Wasana Suyotha
2
,
Poonsuk Prasertsan
2

  1. College of Innovation and Management, Songkhla Rajabhat University, Songkhla, Thailand
  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla, Thailand
BioTechnologia vol. 97(4) C pp. 271-284 C 2016
DOI: https://doi.org/10.5114/bta.2016.64544
Online publish date: 2017/01/13
View full text Get citation
 
PlumX metrics:
Chitinase and β-1,3 glucanase are enzymes that play important roles in the biocontrol of fungal plant pathogens. The effects of environmental conditions and culture medium composition on simultaneous chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-138 were investigated using a conventional (one-factorat- a-time) method and a response surface methodology (RSM), respectively. The optimum cultivation conditions were at pH 7.5 and a temperature of 30EC. The optimized medium (4.88 g @ l!1 chitin, 6.27 g @ l!1 glucose, and 17.05 g @ l!1 malt extract) exhibited 53- and 80-fold increase in the activity of chitinase (0.53 U @ ml!1) and β-1,3 glucanase (8.79 U @ ml!1), respectively, compared to the original medium. The culture filtrate from the original and the optimized medium were partially purified and tested (by agar-well diffusion assay) for their antifungal activities against Rhizoctonia solani PTRRC-9 compared to the chemical fungicides carbendazim® and propiconazole ® (100 μg @ ml!1). The partially purified enzymes from the optimized medium exhibited 4.0-fold stronger antifungal activities against R. solani PTRRC-9 compared to that from the original medium and equal to that of

the chemical fungicide propiconazole® but slightly lower than that of carbendazim®.
keywords:

Streptomyces philanthi , chitinase, β-1,3 glucanases, response surface methodology, rice sheath blight


Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here
Hosted by Termedia sp. z o.o.